GB CRISPR Domesticator
The first step for generating a CRISPR/Cas9 construct is the selection of the genomic target. The genomic target must contain a 20 nucleotides TARGET sequence followed by the PAM sequence (usually NGG). To search your genomic targets minimizing off-targeting you can use online tools such as Benchling or CRISPR-P.
Once you have selected your 20 nucleotides TARGET sequence, you can create your GB-adapted guide RNA part using the web tool below: