Publications and Manuals

If you want to learn more about the GoldenBraid cloning system, please read our publications:

  • Selma S, Bernabé-Orts JM, Vazquez-Vilar M, Diego-Martin B, Ajenjo M, Garcia-Carpintero V, Granell A, Orzaez D. (2019) Strong gene activation in plants with genome-wide specificity using a new orthogonal CRISPR/Cas9-based programmable transcriptional activator. Plant Biotechnol J. 2019 Sep;17(9):1703-1705. doi: 10.1111/pbi.13138.
  • Bernabé-Orts JM, Casas-Rodrigo I, Minguet EG, Landolfi V, Garcia-Carpintero V, Gianoglio S, Vázquez-Vilar M, Granell A, Orzaez D. (2019) Assessment of Cas12a-mediated gene editing efficiency in plants. Plant Biotechnol J. 2019 Oct;17(10):1971-1984. doi: 10.1111/pbi.13113
  • Hernanz-Koers M, Gandía M, Garrigues S, Manzanares P, Yenush L, Orzaez D, Marcos JF. (2018) FungalBraid: A GoldenBraid-based modular cloning platform for the assembly and exchange of DNA elements tailored to fungal synthetic biology. Fungal Genet Biol. 2018 Jul;116:51-61. doi: 10.1016/j.fgb.2018.04.010.
  • Vazquez-Vilar M, Quijano-Rubio A, Fernandez-Del-Carmen A, Sarrion-Perdigones A, Ochoa-Fernandez R, Ziarsolo P, Blanca J, Granell A, Orzaez D. (2017) GB3.0: a platform for plant bio-design that connects functional DNA elements with associated biological data. Nucleic Acids Res. 2017 Feb 28;45(4):2196-2209. doi: 10.1093/nar/gkw1326.
  • Vazquez-Vilar M, Bernabé-Orts JM, Fernandez-Del-Carmen A, Ziarsolo P, Blanca J, Granell A, Orzaez D. (2016) A modular toolbox for gRNA-Cas9 genome engineering in plants based on the GoldenBraid standard. Plant Methods. 2016 Feb 1;12:10. doi: 10.1186/s13007-016-0101-2.
  • Vazquez-Vilar M, Sarrion-Perdigones A, Ziarsolo P, Blanca J, Granell A, Orzaez D. (2015) Software-Assisted Stacking of Gene Modules Using GoldenBraid 2.0 DNA-Assembly Framework. Methods Mol Biol 1284:399-420
  • Sarrion-Perdigones A, Palaci J, Granell A, Orzaez D. (2014) Design and construction of multigenic constructs for plant biotechnology using the GoldenBraid cloning strategy. Methods Mol Biol 1116:133-51
  • Sarrion-Perdigones A, Vazquez-Vilar M, Palací J, Castelijns B, Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D. (2013) GoldenBraid2.0: A comprehensive DNA assembly framework for Plant Synthetic Biology. Plant Physiol 2013 Jul;162(3):1618-31.
  • Sarrion-Perdigones A, Falconi EE, Zandalinas SI, Juárez P, Fernández-del-Carmen A, Granell A, Orzaez D. (2011) GoldenBraid: an iterative cloning system for standardized assembly of reusable genetic modules. PLoS One 6(7): e21622. doi:10.1371/journal.pone.0021622

For a more visual description of the GB system you may also have a look at the following GB.ppt presentations:

Finally, we compiled a GB 2.0 Users Manual where you can find a more didactic description of the system.

Video Tutorials

Video TutorialsThese brief video tutorials provide a practical explanation on how to use the different software tools available in this site:

GB Domesticator TutorialGB Domesticator Tutorial

TU Assembler TutorialTU Assembler Tutorial

Binary Assembler TutorialBinary Assembler Tutorial