Publications and Manuals

If you want to learn more about the GoldenBraid cloning system, please read our publications:

  • Vazquez-Vilar M, Sarrion-Perdigones A, Ziarsolo P, Blanca J, Granell A, Orzaez D. (2015) Software-Assisted Stacking of Gene Modules Using GoldenBraid 2.0 DNA-Assembly Framework. Methods Mol Biol 1284:399-420
  • Sarrion-Perdigones A, Palaci J, Granell A, Orzaez D. (2014) Design and construction of multigenic constructs for plant biotechnology using the GoldenBraid cloning strategy. Methods Mol Biol 1116:133-51
  • Sarrion-Perdigones A, Vazquez-Vilar M, Palací J, Castelijns B, Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D. (2013) GoldenBraid2.0: A comprehensive DNA assembly framework for Plant Synthetic Biology. Plant Physiol Epub ahead of print, doi: 10.​1104/​pp.​113.​217661
  • Sarrion-Perdigones A, Falconi EE, Zandalinas SI, Juárez P, Fernández-del-Carmen A, Granell A, Orzaez D. (2011) GoldenBraid: an iterative cloning system for standardized assembly of reusable genetic modules. PLoS One 6(7): e21622. doi:10.1371/journal.pone.0021622

For a more visual description of the GB system you may also have a look at the following GB.ppt presentations:

Finally, we compiled a GB 2.0 Users Manual where you can find a more didactic description of the system.

Video Tutorials

Video TutorialsThese brief video tutorials provide a practical explanation on how to use the different software tools available in this site:

GB Domesticator TutorialGB Domesticator Tutorial

TU Assembler TutorialTU Assembler Tutorial

Binary Assembler TutorialBinary Assembler Tutorial