GB polycistronic gRNA design for SpCas9

With these tools you can design and assemble your own polycistronic gRNA constructs for gene editing with Cas9.

Polycistronic gRNAs assembly with GB involves three consecutive steps: (1) adaptation of your own protospacer sequence to the GB standard, (2) cloning in the pUPD2 and (3) assembly of the polycistronic TU with the selected PolIII promoter.

Take a look to the GB polycistronic structures before proceeding to Step 2 and click here for detailed instructions.