GB polycistronic gRNA design for Sp(d)Cas9

With these tools you can design and assemble your own PolII polycistronic gRNA constructs for gene regulation with Cas9.

Polycistronic gRNAs assembly involves three consecutive steps: (1) adaptation of your own protospacer sequence to the GB standard, (2) cloning in the pUPD2 and (3) assembly of the polycistronic TU with the selected PolII promoter.